Primitive lymphohematopoietic precursor cell lines generated in culture from day 7 early-mid-primitive streak stage mouse embryo.

The in vitro culture of primitive-streak-stage mouse embryos.

The in vitro growth and morphogenesis of mouse embryos from early primitive-streak stage to early-somite stage is described. The embryo culture method employs a static culture system, a conventional chemically defined medium (Dulbecco's modified Eagle's medium), supplemented with additional glucose, glutamine and suitably prepared serum. The method of serum preparation is important for successf...

Cinemicrographic study of the cell movement in the primitive-streak-stage mouse embryo.

Migration of the mesoderm cells in the primitive-streak-stage mouse embryo was directly studied by cinemicrography using whole embryo culture and Nomarski differential interference contrast optics. Relative transparency and small size of the early mouse embryos enabled direct observation of the individual cells and their cell processes. Seven-day-old mouse embryos were isolated and cultured in ...

SnapShot: Mouse Primitive Streak

The development of 12th to 14th day foetuses following reimplantation of pre- and early-primitive-streak-stage mouse embryos.

This paper describes a technique for transplanting early postimplantation mouse embryos from their own implantation site to a decidua in another pregnant mouse. Evidence is provided that this procedure is compatible with their continued development. At a low frequency both 6th and 7th day embryos can re-establish themselves and continue apparently normal development and placentation for at leas...

A model of primitive streak initiation in the chick embryo.

Initiation of the primitive streak in avian embryos provides a well-studied example of a pattern-forming event that displays a striking capacity for regulation. The mechanisms underlying the regulative properties are, however, poorly understood and are not easily accounted for by traditional models of pattern formation, such as reaction-diffusion models. In this paper, we propose a new activato...